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Rabbit anti-Chicken IgG (F(ab')2 Region) Antibody (Alkaline Phosphatase (AP))

Rockland 603-4504 ELISA, IHC, WB, DB Polyclonal IgG Alkaline Phosphatase (AP)
Rockland
Catalog No. ABIN101060
Supplier Product No.: 603-4504
  • Target See all IgG products
    IgG
    Binding Specificity
    • 2403
    • 511
    • 416
    • 148
    • 129
    • 128
    • 67
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 1
    • 1
    F(ab')2 Region
    Reactivity
    • 720
    • 692
    • 594
    • 465
    • 393
    • 240
    • 208
    • 176
    • 144
    • 137
    • 112
    • 106
    • 105
    • 101
    • 82
    • 58
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 8
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Chicken
    Host
    • 1761
    • 1548
    • 569
    • 238
    • 112
    • 111
    • 32
    • 31
    • 21
    • 18
    • 15
    • 11
    • 11
    Rabbit
    Clonality
    • 3389
    • 175
    • 1
    Polyclonal
    Conjugate
    • 583
    • 418
    • 414
    • 378
    • 273
    • 262
    • 235
    • 119
    • 106
    • 73
    • 67
    • 64
    • 61
    • 58
    • 58
    • 50
    • 44
    • 43
    • 40
    • 39
    • 39
    • 37
    • 35
    • 32
    • 32
    • 31
    • 30
    • 29
    • 29
    • 29
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 27
    • 26
    • 26
    • 25
    • 22
    • 21
    • 19
    • 17
    • 17
    • 16
    Alkaline Phosphatase (AP)
    Application
    • 2032
    • 1981
    • 1835
    • 1784
    • 983
    • 920
    • 896
    • 486
    • 419
    • 234
    • 234
    • 151
    • 99
    • 84
    • 65
    • 52
    • 51
    • 30
    • 28
    • 25
    • 20
    • 15
    • 11
    • 9
    • 8
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
    Supplier Product No.
    603-4504
    Supplier
    Rockland
    Purpose
    Chicken IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Chicken IgG, Chicken IgG F(ab')2 and Chicken Serum.  No reaction was observed against Chicken IgG F(c).
    Characteristics
    Anti-Chicken IgG F(c) Antibody detects specifically Chicken IgG F(c). It is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .
    Purification
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    Sterility
    Sterile filtered
    Immunogen
    Optional[Immunogen]: Chicken IgG F(ab')2 fragment
    Isotype
    IgG
  • Application Notes
    Application Note: Anti-Chicken IgG F(ab')2 Alkaline Phosphatase antibody has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:3,500 Other: User Optimized
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background
    Anti-Chicken IgG F(ab')2 Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Chicken IgG F(c) will not bind Chicken IgG F(ab')2 Molecules.
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