Rabbit anti-Cow IgG (Fc Region) Antibody - Preadsorbed
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- Target See all IgG products
- IgG
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Binding Specificity
- Fc Region
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Reactivity
- Cow
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Host
- Rabbit
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Clonality
- Polyclonal
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- IgG F(c)
- Cross-Reactivity
- Cow (Bovine)
- Characteristics
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Anti-Bovine IgG F(c) antibody generated in rabbit detects specifically bovine IgG. This secondary antibody anti-Bovine is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm - Purification
- Preadsorption: Solid phase absorption
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: Anti-Bovine IgG F(c) was produced by repeated immunization with bovine IgG F(c) fragment in rabbit.
Immunogen Type: Native Protein
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Bovine IgG F(c) Antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 2.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
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Synonyms: rabbit anti-Bovine IgG F(c) Antibody, Rb-a-Bovine IgG F(c), Bovine IgG F(c) Antibody in Rabbit, Rabbit IgG F(c) Secondary Antibody.
Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(c) region, recognized by high-affinity Fc receptor proteins.
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