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Rabbit anti-Cow IgG (Fc Region) Antibody - Preadsorbed

ELISA, IHC, WB Polyclonal IgG unconjugated
Catalog No. ABIN100938
  • Target See all IgG products
    IgG
    Binding Specificity
    • 2382
    • 511
    • 424
    • 207
    • 132
    • 129
    • 95
    • 29
    • 28
    • 28
    • 22
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    Fc Region
    Reactivity
    • 724
    • 699
    • 602
    • 477
    • 403
    • 243
    • 212
    • 175
    • 147
    • 138
    • 113
    • 112
    • 107
    • 102
    • 82
    • 59
    • 47
    • 28
    • 28
    • 16
    • 12
    • 10
    • 8
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cow
    Host
    • 1778
    • 1575
    • 572
    • 241
    • 112
    • 111
    • 33
    • 31
    • 21
    • 19
    • 15
    • 11
    • 11
    • 1
    Rabbit
    Clonality
    • 3434
    • 175
    • 1
    Polyclonal
    Application
    • 1952
    • 1838
    • 1806
    • 1519
    • 934
    • 910
    • 652
    • 478
    • 380
    • 236
    • 236
    • 234
    • 106
    • 103
    • 86
    • 67
    • 53
    • 52
    • 31
    • 28
    • 25
    • 21
    • 18
    • 16
    • 11
    • 9
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    IgG F(c)
    Cross-Reactivity
    Cow (Bovine)
    Characteristics
    Anti-Bovine IgG F(c) antibody generated in rabbit detects specifically bovine IgG. This secondary antibody anti-Bovine is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Anti-Bovine IgG F(c) was produced by repeated immunization with bovine IgG F(c) fragment in rabbit.

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Bovine IgG F(c) Antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    2.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background

    Synonyms: rabbit anti-Bovine IgG F(c) Antibody, Rb-a-Bovine IgG F(c), Bovine IgG F(c) Antibody in Rabbit, Rabbit IgG F(c) Secondary Antibody.

    Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(c) region, recognized by high-affinity Fc receptor proteins.

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