Rabbit anti-Cow IgG (F(ab')2 Region) Antibody

Catalog No. ABIN100920

Product Details

Target: IgG

Binding Specificity: F(ab')2 Region

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgG F(ab')2

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Bovine IgG, Bovine IgG F(ab')2 and Bovine Serum.  No reaction was observed against Bovine IgG F(c).

Immunogen:

Immunogen: Bovine IgG F(ab')2 fragment

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 75.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: RT,4 °C,-20 °C

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: rabbit anti-Bovine IgG F(ab')2 fragment Antibody, rabbit anti-Bovine IgG Fab2 fragment Antibody, rabbit anti Bovine IgG Fab2

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.