Goat anti-Human IgA, IgG, IgM (Heavy & Light Chain) Antibody (FITC) - Preadsorbed
-
- Target See all IgA, IgG, IgM products
- IgA, IgG, IgM
-
Binding Specificity
- Heavy & Light Chain
-
Reactivity
- Human
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- FITC
-
Application
- Flow Cytometry (FACS), Fluorescence Microscopy (FM), FLISA
- Specificity
- IgG IgA IgM
- Characteristics
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- Preadsorption: immunoaffinity chromatography using antigens coupled to agarose beads
- Immunogen
-
Immunogen: Human IgG, IgA and IgM whole molecules
- Isotype
- IgG
- Labeling Ratio
- 4.9
-
-
- Application Notes
-
Application Note: Anti-Human IgG IgA IgM (H&L) Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- Comment
-
Excitation/Emission wavelength: 494 nm/514 nm
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 2.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Product is photosensitive and should be protected from light.
- Storage
- RT,4 °C,-20 °C
- Expiry Date
- 12 months
-
- Target
- IgA, IgG, IgM
- Alternative Name
- IgG IgA IgM (IgA, IgG, IgM Products)
- Target Type
- Antibody
- Background
-
Synonyms: Goat Anti-Human IgG IgA IgM Antibody fluorescein Conjugated, Goat Anti-Human IgG IgA IgM Antibody fluorescein Conjugated, Goat Anti-Human IgGAM Antibody FITC Conjugated
Background: Anti-Human IgG IgA IgM (H&L) Fluorescein Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsinize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
-