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Rabbit anti-Human IgA Antibody

Rockland 609-4106 ELISA, IHC, WB Polyclonal IgG unconjugated
Rockland
Catalog No. ABIN100722
Supplier Product No.: 609-4106
  • Target See all IgA products
    IgA
    Reactivity
    • 179
    • 59
    • 44
    • 16
    • 15
    • 13
    • 13
    • 12
    • 11
    • 11
    • 10
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 202
    • 100
    • 53
    • 17
    • 14
    • 6
    • 2
    Rabbit
    Clonality
    • 300
    • 67
    Polyclonal
    Application
    • 189
    • 133
    • 123
    • 106
    • 102
    • 101
    • 61
    • 47
    • 41
    • 34
    • 28
    • 26
    • 23
    • 18
    • 16
    • 16
    • 14
    • 7
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Supplier Product No.
    609-4106
    Supplier
    Rockland
    Purpose
    Human IgA (alpha chain) Antibody
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgA and Human Serum.  No reaction was observed against other human heavy or light chain proteins.
    Characteristics
    Anti-Human IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind human IgG F(c) will not bind human IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
    Purification
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose followed by solid phase adsorption(s).
    Sterility
    Sterile filtered
    Immunogen
    Optional[Immunogen]: Human IgA alpha heavy chain
    Isotype
    IgG
  • Application Notes
    Application Note: Anti-Human IgA Antibody is tested for ELISA and western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Immunohistochemistry Dilution: 1:250 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    2.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    , Preservative:0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
    Expiry Date
    12 months
  • Target
    IgA
    Abstract
    IgA Products
    Target Type
    Antibody
    Background
    Anti-Human IgA Antibody generated in rabbit detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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