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Reticulon 1C antibody

NSP-C Reactivity: Human, Rat ICC, FACS, IHC (fro), IHC, WB Host: Mouse Monoclonal RNL-4 unconjugated
Catalog No. ABIN335376
  • Target
    Reticulon 1C (NSP-C)
    Reactivity
    Human, Rat
    Host
    Mouse
    Clonality
    Monoclonal
    Application
    Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    Human and rat.
    Purification
    Purified
    Immunogen
    RNL-4 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells, with spleen cells from a BALB/c mouse immunized with a synthetic peptide encompassing the unique 20 N-terminal amino acid sequence of Reticulon-1C.
    Clone
    RNL-4
    Isotype
    IgG1
  • Application Notes
    RNL-4 recognizes an epitope located within the first 20 amino acids of Reticulon-1C (NSP-C). RNL-4 reacts with peripheral nerves and ganglia of various tissues and cross-reacts with smooth muscle cells and myoepithelium. In the central nervous system it reacts with the neurohypophysis and pars intermedia of the pituitary gland, and a weak diffuse staining was observed in neurons of the granular and molecular layer of the cerebellar cortex, while glial cells, cerebellar medulla and Purkinje cells are negative. Reticulon-1 has been found to indicate neuronal differentiation and to be downregulated in neurological pathologies. RNL-4 is useful for immunocytochemistry, immunohistochemistry on frozen tissues, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:50 - 1:100 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting applications.
    Restrictions
    For Research Use only
  • Storage
    4 °C
  • Kim, Yoo, Broers, Cairns, Lubec: "Neuroendocrine-specific protein C, a marker of neuronal differentiation, is reduced in brain of patients with Down syndrome and Alzheimer's disease." in: Biochemical and biophysical research communications, Vol. 276, Issue 1, pp. 329-34, (2000) (PubMed).

    Hens, Nuydens, Geerts, Senden, Van de Ven, Roebroek, van de Velde, Ramaekers, Broers: "Neuronal differentiation is accompanied by NSP-C expression." in: Cell and tissue research, Vol. 292, Issue 2, pp. 229-37, (1998) (PubMed).

    Senden, Timmer, de Bruïne, Wagenaar, Van de Velde, Roebroek, Van de Ven, Broers, Ramaekers: "A comparison of NSP-reticulons with conventional neuroendocrine markers in immunophenotyping of lung cancers." in: The Journal of pathology, Vol. 182, Issue 1, pp. 13-21, (1997) (PubMed).

    Senden, Timmer, Boers, van de Velde, Roebroek, Van de Ven, Broers, Ramaekers: "Neuroendocrine-specific protein C (NSP-C): subcellular localization and differential expression in relation to NSP-A." in: European journal of cell biology, Vol. 69, Issue 3, pp. 197-213, (1996) (PubMed).

  • Target
    Reticulon 1C (NSP-C)
    Alternative Name
    Reticulon-1C / NSP-C
    Background
    Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSP-A), Reticulon-1B (NSP-B), and Reticulon-1C (NSP-C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).
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