dicots and monocots (target sequence is only weakly conserved in Oryza sativa)
Characteristics
Expected / apparent Molecular Weight of the Antigene: 18 / 17-18 for Arabidopsis thaliana
Purification
serum
Immunogen
KLH-conjugated synthetic peptide derived from PsaL protein sequence from Arabidopsis thaliana (At4g12800). This sequence is well conserved in most mono- and dicots but not in Physcomitrella patens.
Application Notes
1: 1000 with standard ECL (WB)
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 200 μL of sterile water
Handling Advice
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage
-20 °C
Storage Comment
store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Target
Psal
(psi-L Subunit of Photosystem I (Psal))
Background
AGI Code: At4g12800 PsaL (PSI-L) is a conserved subunit of type I photosynthetic reaction centers (Photosystem I, PSI). PSI is an integral membrane multi-protein complex that catalyzes the electron transfer from plastocyanin (or cytochrome c6) to ferredoxin (or flavodoxin). Psa-L is binding pigments and has been shown to be involved in trimerization of PSI in cyanobacteria (but not in plants) and bind pigments in plants and cyanobacteria.In plants and algae Psa-L is nuclear encoded and imported post-translationally into the chloroplast where it inserts into the thylakoid membrane.
Molecular Weight
expected: 18 kDa, apparent: 17-18 for Arabidopsis thaliana