psaB antibody
psaB
Reactivity: Arabidopsis thaliana, Barley, Chlamydomonas reinhardtii, Nicotiana tabacum
WB
Host: Rabbit
Polyclonal
unconjugated
1 image
(4 references)-
- Target
- psaB (psi-B Core Subunit of Photosystem I (psaB))
- Reactivity
- Arabidopsis thaliana, Barley, Chlamydomonas reinhardtii, Nicotiana tabacum
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Western Blotting (WB)
- Cross-Reactivity (Details)
- Peptide target used to elicit this antibody is well conserved in all photoautotrophs except some cyanobacteria, some red algae and Cyanophora paradoxa, which contain a conserved substitution of a valine to an isoleucine. The performance of the antibodies has been confirmed against taxa containing both the valine andisoleucine variants.
- Characteristics
- Expected / apparent Molecular Weight of the Antigene: 82.7 / 55-60 kDa
- Purification
- serum
- Immunogen
- KLH-conjugated synthetic peptide derived from known PsaB sequences including Arabidopsis thaliana P56767, AtCg00340
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- Application Notes
- Recommended Dilution: 1 : 1000 with standard ECL (WB).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 200 µL, of sterile water.
- Handling Advice
- Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
- Storage
- -20 °C
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: "Arabidopsis tic62 trol mutant lacking thylakoid-bound ferredoxin-NADP+ oxidoreductase shows distinct metabolic phenotype." in: Molecular plant, Vol. 7, Issue 1, pp. 45-57, (2014) (PubMed).
: "The bacterial-type [4Fe-4S] ferredoxin 7 has a regulatory function under photooxidative stress conditions in the cyanobacterium Synechocystis sp. PCC 6803." in: Biochimica et biophysica acta, Vol. 1837, Issue 8, pp. 1293-304, (2014) (PubMed).
: "LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, (2012) (PubMed).
: "The plastid genome-encoded Ycf4 protein functions as a nonessential assembly factor for photosystem I in higher plants." in: Plant physiology, Vol. 159, Issue 2, pp. 579-91, (2012) (PubMed).
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: "Arabidopsis tic62 trol mutant lacking thylakoid-bound ferredoxin-NADP+ oxidoreductase shows distinct metabolic phenotype." in: Molecular plant, Vol. 7, Issue 1, pp. 45-57, (2014) (PubMed).
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- Target
- psaB (psi-B Core Subunit of Photosystem I (psaB))
- Synonyms
- PSI P700 apoprotein A2 antibody, psaB antibody
- Background
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AGI Code: ATCG00340
Photosystem I (PSI) of chloroplasts is a multisubunit membrane-protein complex that catalyzes the electron transfer from the reduced plastocyanin (or cytochrome c6) in the thylakoid lumen to the oxidized ferredoxin (or flavodoxin) in the chloroplast stroma. PsaB is a core protein of PSI complex. Synonymes: Photosystem I P700 chlorophyll a apoprotein A2, PSI-B - Molecular Weight
- expected: 82.7 kDa, apparent: 55-60 kDa
- UniProt
- P56767
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