CCL25 Protein

Catalog No. ABIN988253

Product Details

Target: CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Sequence: QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAA IFYLPKRHRK VCGNPKSREV QRAMKLLDAR NKVFAKLHHN MQTFQAGPHA VKKLSSGNSK LSSSKFSNPI SSSRKNVSLL ISANSG

Characteristics: Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human monocytes is less than 10 ng/ml, corresponding to a specific activity of >, 1.0 × 105 IU/mg.

Purity: > 97 % by SDS-PAGE and HPLC analyses.

Endotoxin Level: Level Less than 1EU/µg of rHuTECK/CCL25 as determined by LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at

Storage: 4 °C

Target Details

Target: CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))

Alternative Name: TECK/CCL25 (CCL25 Products)

Synonyms: CCL25 Protein, Ckb15 Protein, SCYA25 Protein, TECK Protein, A130072A22Rik Protein, AI852536 Protein, CKb15 Protein, Scya25 Protein, C-C motif chemokine ligand 25 Protein, C-C motif chemokine 25 Protein, chemokine (C-C motif) ligand 25 Protein, Ccl25 Protein, CCL25 Protein, ccl25 Protein

Background: CCL25 (thymus expressed chemokine) is a novel CC chemokine that is distantly related (approximately 20% amino acid sequence identity) to other CC chemokines. Mouse CCL25 cDNA has also been cloned and shown to encode a 144 aa protein that exhibits 49% aa sequence identity to human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. Recombinant human and mouse CCL25 have been shown to be chemotactic for activated macrophages, dendritic cells and thymocytes. CCL25 signals through the CCR9 receptor. Synonym: TECK/CCL25, Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.

Molecular Weight: 14.2 kDa, a single, non-glycosylated polypeptide chain containing 127 amino acids.