CCL15 Protein

Catalog No. ABIN988169

Product Details

Target: CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Sequence: QFTNDAETEL MMSKLPLENP VVLNSFHFAA DCCTSYISQS IPCSLMKSYF ETSSECSKPG VIFLTKKGRQ VCAKPSGPGV QDCMKKLKPY S

Characteristics: Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human T-lymphocytes is less than 10 ng/ml, corresponding to a specific activity of >, 1.0 × 105 IU/mg.

Purity: > 97 % by SDS-PAGE and HPLC analyses.

Endotoxin Level: Level Less than 1EU/µg of rHuMIP-5/CCL15 as determined by LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage: 4 °C

Target Details

Target: CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))

Alternative Name: MIP-5/CCL15 (CCL15 Products)

Synonyms: HCC-2 Protein, HMRP-2B Protein, LKN-1 Protein, LKN1 Protein, MIP-1 delta Protein, MIP-1D Protein, MIP-5 Protein, MRP-2B Protein, NCC-3 Protein, NCC3 Protein, SCYA15 Protein, SCYL3 Protein, SY15 Protein, C-C motif chemokine ligand 15 Protein, CCL15 Protein

Background: CCL15, a new human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 amino acids that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1γ, respectively. The gene for MIP-5 is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-5 is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells. Synonym: MIP-5/CCL15, Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 100mM NaCl.

Molecular Weight: 10.1 kDa, a single non-glycosylated polypeptide chain containing 92 amino acids.