CCL18 Protein
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- Target See all CCL18 Proteins
- CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Sequence
- AQVGTNKELC CLVYTSWQIP QKFIVDYSET SPQCPKPGVI LLTKRGRQIC ADPNKKWVQK YISDLKLN
- Characteristics
- Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human T-lymphocytes is less than 10 ng/ml, corresponding to a specific activity of >, 1.0 × 105 IU/mg.
- Purity
- > 97 % by SDS-PAGE and HPLC analyses.
- Endotoxin Level
- Level Less than 1EU/µg of rHuMIP-4/CCL18 as determined by LAL method
- Top Product
- Discover our top product CCL18 Protein
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Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18) (Active) protein
CCL18 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18) protein (His tag)CCL18 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18) (AA 1-89) protein (GST tag)CCL18 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18) (AA 22-89) protein (His tag)CCL18 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18) (AA 21-89) protein (His tag)CCL18 Origin: Human Host: Yeast Recombinant > 90 % ELISA
Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18) protein (Myc-DYKDDDDK Tag)CCL18 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
- Storage
- 4 °C
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- Target
- CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))
- Alternative Name
- MIP-4/CCL18 (CCL18 Products)
- Background
- CCL18, is a novel CC chemokine that is highly homologous to MIP-1ª (61% amino acid sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN-γ. CCL18 mRNA is also expressed by GM-CSF/IL-4-induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells, but not monocytes or neutrophils. Synonym: MIP-4/CCL18, Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 100mM NaCl.
- Molecular Weight
- 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.
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