CCL3 Protein

Catalog No. ABIN988157

Product Details

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Sequence: ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELS

Characteristics: Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human monocytes is less than 10 ng/ml, corresponding to a specific activity of >, 1.0 × 105 IU/mg.

Purity: > 96 % by SDS-PAGE and HPLC analyses.

Endotoxin Level: Level Less than 1EU/µg of rHu MIP-1 alpha /CCL3 as determined by LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage: 4 °C

Target Details

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Alternative Name: MIP-1 alpha/CCL3 (CCL3 Products)

Synonyms: MIP-1alpha Protein, G0S19-1 Protein, LD78ALPHA Protein, MIP-1-alpha Protein, MIP1A Protein, SCYA3 Protein, MIP-1a Protein, Scya3 Protein, AI323804 Protein, LD78alpha Protein, MIP1-(a) Protein, MIP1-alpha Protein, Mip1a Protein, CCL3L1 Protein, CCL3L3 Protein, chemokine (C-C motif) ligand 3 Protein, C-C motif chemokine ligand 3 Protein, CCL3 Protein, Ccl3 Protein

Background: Both MIP-1 alpha and MIP-1 beta are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ, with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1 alpha and MIP-1 beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and mouse MIP-1 alpha and MIP-1 beta are active on human and mouse hematopoietic cells. Synonym: MIP-1 alpha/CCL3, Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 100mM NaCl.

Molecular Weight: Approximately 7.8 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy