MICB Protein

Catalog No. ABIN988150

Product Details

Target: MICB (MHC Class I Polypeptide-Related Sequence B (MICB))

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA

Characteristics: Measured by its ability to bind MICB antibody in a ELISA.

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Endotoxin Level: Level Less than 1EU/µg of rHuMIC-B as determined by LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

Storage: 4 °C

Target Details

Target: MICB (MHC Class I Polypeptide-Related Sequence B (MICB))

Alternative Name: MIC-B (MICB Products)

Synonyms: PERB11.2 Protein, MHC class I polypeptide-related sequence B Protein, MHC class I-related protein-like Protein, MICB Protein, LOC512825 Protein

Background: MIC-B (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MIC-A, shares 85% amino acid identity with MIC-B. These 2 proteins are distantly related to the MHC class I proteins. MIC-A and MIC-B (MIC-A/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with beta2-microglobulin. The genes encoding MIC-A/B are found within the major histocompatibility complex on human chromosome 6. The MIC-B locus is polymorphic with more than 15 recognized human alleles. MIC-A/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MIC-A/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, γδ T cells, and CD8+ abeta T cells. Recognition of MIC-A/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MIC-A/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MIC-A/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response. Synonym:. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Molecular Weight: Approximately 37 kDa, 326 amino acid residues containing the extracellular domain of mature human MICB (amino acid residues Ala23 – Tyr312).100µg /1000µg

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response