MICA Protein

Catalog No. ABIN988148

Product Details

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA

Sequence: MSYYHHHHHH DYDIPTTENL YFQGAMAPEF EPHSLRYNLT VLSWDGSVQS GFLTEVHLDG QPFLRCDRQK CRAKPQGQWA EDVLGNKTWD RETRDLTGNG KDLRMTLAHI KDQKEGLHSL QEIRVCEIHE DNSTRSSQHF YYDGELFLSQ NLETKEWTMP QSSRAQTLAM NVRNFLKEDA MKTKTHYHAM HADCLQELRR YLKSGVVLRR TVPPMVNVTR SEASEGNITV TCRASGFYPW NITLSWRQDG VSLSHDTQQW GDVLPDGNGT YQTWVATRIC QGEEQRFTCY MEHSGNHSTH PVPSGKVLVL QSHW

Characteristics: Measured by its ability to bind MICA antibody in a ELISA.

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Endotoxin Level: Level Less than 1EU/µg of rHuMIC-A as determined by LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage: 4 °C

Target Details

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Alternative Name: MIC-A (MICA Products)

Synonyms: MIC-A Protein, PERB11.1 Protein, MHC class I polypeptide-related sequence A Protein, MICA Protein

Background: MIC-A (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, γδ T cells, and CD8+ ªbeta T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. Synonym: MIC-A, Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Molecular Weight: Approximately 36.5 kDa, a single non-glycosylated polypeptide chain containing 315 amino acids.

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response