CCL8 Protein

Catalog No. ABIN988134

Product Details

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Sequence: GPDKAPVTCC FHVLKLKIPL RVLKSYERIN NIQCPMEAVV FQTKQGMSLC VDPTQKWVSE YMEILDQKSQ ILQ

Characteristics: Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human monocytes is less than 100 ng/ml, corresponding to a specific activity of >, 1.0 × 104 IU/mg.

Purity: > 97 % by SDS-PAGE and HPLC analyses.

Endotoxin Level: Level Less than 1EU/µg of rMuMCP-2/CCL8 as determined by LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage: 4 °C

Target Details

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Alternative Name: MCP-2/CCL8 (CCL8 Products)

Synonyms: HC14 Protein, MCP-2 Protein, MCP2 Protein, SCYA10 Protein, SCYA8 Protein, 1810063B20Rik Protein, AB023418 Protein, Mcp2 Protein, Scya8 Protein, C-C motif chemokine ligand 8 Protein, chemokine (C-C motif) ligand 8 Protein, C-C motif chemokine 8 Protein, monocyte chemoattractant protein-2 precursor Protein, CCL8 Protein, Ccl8 Protein, LOC101119572 Protein, MCP-2 Protein

Background: MCP-2 and MCP-3 are two recently identified monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2.Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils. Synonym: MCP-2/CCL8, Mouse. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.

Molecular Weight: 8.5 kDa, a single, non-glycosylated polypeptide chain containing 74 amino acids.