IL-8 Protein (AA 3-79)

Catalog No. ABIN988103

Product Details

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Protein Type: Recombinant

Protein Characteristics: AA 3-79

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95.0 %, as determined by the following methods: (a) RP-HPLC analysis (b) Reducing and non-reducing SDS-PAGE silver-stained gel analysis

Endotoxin Level: Level Less than 0.1ng/µg (1 IEU/µg) determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that lyophilized Recombinant Human Interleukin-8 (IL-8)/77 be reconstituted in sterile 18 Mohm-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Specificity Recombinant Human Interleukin-8 (IL-8)/77 is fully biologically active when compared to standard. The ED50, as determined by its chemotaxis of hCXCR-2 transfected mouse BaF/3 cells, is less than 2 ng/ml, corresponding to a specific activity of 5.0×105 IU/mg.

Storage: -20 °C

Target Details

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Alternative Name: Interleukin-8 (IL-8) (IL8 Products)

Synonyms: CXCL8 Protein, GCP-1 Protein, GCP1 Protein, LECT Protein, LUCT Protein, LYNAP Protein, MDNCF Protein, MONAP Protein, NAF Protein, NAP-1 Protein, NAP1 Protein, IL-8 Protein, AMCF-I Protein, il8 Protein, IL8 Protein, si:dkey-151b16.2 Protein, cxcli2 Protein, cxcl8 Protein, gcp-1 Protein, il-8 Protein, lynap Protein, mdncf Protein, monap Protein, nap-1 Protein, C-X-C motif chemokine ligand 8 Protein, interleukin 8 Protein, chemokine (C-X-C motif) ligand 8a Protein, chemokine (C-X-C motif) ligand 1-like Protein, chemokine (C-X-C motif) ligand 8 L homeolog Protein, CXCL8 Protein, il8 Protein, cxcl8a Protein, IL8 Protein, Cxcl8 Protein, LOC422654 Protein, cxcl8.L Protein

Background: Interleukin-8 (IL-8) belongs to the neutrophil-specific CXC family of chemokines. It is one of the initial cytokines released from a variety of cell types, including T cells, endothelial cells and fibroblasts, in response to an inflammatory stimulus and acts by recruiting neutrophils, T-cells and basophils to the site of inflammation. Elevated Interleukin-8 (IL-8) levels are associated with the onset of a variety of disease states. Human Interleukin-8 (IL-8) (3-79) , produced in E. coli, is a single, non-glycosylated polypeptide chain containing 77 amino acids and having a molecular mass of 8,904 Da. Synonym: rHu IL-8 (3-79). Formulation: The protein was lyophilized after extensive dialysis against PBS, pH7.4.

Molecular Weight: 9,000 Da+/-10%, determined by reduced SDS-PAGE

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy