IL-22 Protein

Catalog No. ABIN988063

Product Details

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 97.0 %, as determined by the following methods: (a) RP-HPLC analysis (b) Reducing and non-reducing SDS-PAGE silver stained gel analysis

Endotoxin Level: Level The endotoxin level of Recombinant Mouse Interleukin-22 (IL-22) is below 0.1 ng/µg (1 IEU/µg), as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that reconstitute the lyophilized Mouse Interleukin-22 (IL-22) be reconstituted in sterile 18 Mohm-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Specificity Recombinant Mouse Interleukin-22 (IL-22) is fully biologically active relative to standard. The ED50, as determined by its ability to induce IL-10 secretion in Colo205 cells, is less than 0.5 ng/ml, corresponding to a specific activity of 2.0×106 IU/mg.

Storage: -20 °C

References

Ki, Park, Zheng, Morales-Ibanez, Kolls, Bataller, Gao: "Interleukin-22 treatment ameliorates alcoholic liver injury in a murine model of chronic-binge ethanol feeding: role of signal transducer and activator of transcription 3." in: Hepatology (Baltimore, Md.), Vol. 52, Issue 4, pp. 1291-300, (2010) (PubMed).

Target Details

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Alternative Name: Interleukin-22 (IL-22) (IL22 Products)

Synonyms: IL-21 Protein, IL-22 Protein, IL-D110 Protein, IL-TIF Protein, ILTIF Protein, TIFIL-23 Protein, TIFa Protein, zcyto18 Protein, IL-22a Protein, ILTIFa Protein, Iltif Protein, RGD1561292 Protein, interleukin-22 Protein, interleukin 22 Protein, IL22 Protein, il22 Protein, Il22 Protein

Background: Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse Interleukin-22 (IL-22) cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. Interleukin-22 (IL-22) has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. Interleukin-22 (IL-22) is produced by normal mouse T cells upon Con activation. Mouse Interleukin-22 (IL-22) expression is also induced in various organs upon lipopolysaccharide injection, suggesting that Interleukin-22 (IL-22) may be involved in inflammatory responses. The functional Interleukin-22 (IL-22) receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10Rbeta (previously known as CRF2-4), belonging to the class II cytokine receptor family. Mouse Interleukin-22 (IL-22), produced in E. coli, is a single, non-glycosylated polypeptide chain containing 147 amino acids and having a molecular mass of 16,700 Da. Synonym: IL-22 (Mouse), rmIL-22. Formulation: The protein was lyophilized after extensive dialysis against 20mM PB, pH 6.5, 300mM NaCl buffer.

Molecular Weight: 16,700 Da