IL-2 Protein

Catalog No. ABIN988052

Product Details

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Sequence: APTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ LLMDLQELLS RMENYRNLKL PRMLTFKFYL PKQATELKDL QCLEDELGPL RHVLDLTQSK SFQLEDAENF ISNIRVTVVK LKGSDNTFEC QFDDESATVV DFLRRWIAFC QSIISTSP

Characteristics: The ED50 as determined by the dose dependent stimulation of murine CTLL-2 cells is < 0.2 ng/ml, corresponding to a specific activity of > 5.0 x 106 units/mg.

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Endotoxin Level: Level Less than 1EU/µg of rMuIL-2 as determined by LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at

Storage: 4 °C

References

Phuan, Ratelade, Rossi, Tradtrantip, Verkman: "Complement-dependent cytotoxicity in neuromyelitis optica requires aquaporin-4 protein assembly in orthogonal arrays." in: The Journal of biological chemistry, Vol. 287, Issue 17, pp. 13829-39, (2012) (PubMed).

Brewin, Mancao, Straathof, Karlsson, Samarasinghe, Amrolia, Pule: "Generation of EBV-specific cytotoxic T cells that are resistant to calcineurin inhibitors for the treatment of posttransplantation lymphoproliferative disease." in: Blood, Vol. 114, Issue 23, pp. 4792-803, (2009) (PubMed).

Target Details

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: Interleukin-2 (IL-2 ) (IL2 Products)

Synonyms: IL2 Protein, IL-2 Protein, il2 Protein, TCGF Protein, lymphokine Protein, Il-2 Protein, POIL2 Protein, interleukin 2 Protein, interleukin-15 Protein, IL2 Protein, il2 Protein, LOC100303688 Protein, Il2 Protein

Background: Mature mouse IL-2 shares 56% and 73% aa sequence identity with human and rat IL-2, respectively. It shows strain-specific heterogeneity in an N-terminal region that contains a poly-glutamine stretch. Mouse and human IL-2 exhibit cross-species activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes . The 55 kDa IL-2 Rªis specific for IL-2 and binds with low affinity. The 75 kDa IL-2 Rbeta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chainγc/IL-2 Rγ, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 Rbetaandγc. It drives resting T cells to proliferate and induces IL-2 and IL-2 Rªsynthesis. It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes . IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells. Synonym: Interleukin-2 ( IL-2 ), Mouse. Formulation: Lyophilized from a 0.2µm filtered solution in PBS, pH 7.4.

Molecular Weight: Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 149 amino acids.

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation