Hirudin Protein
Origin: Leech
Host: Yeast (Pichia pastoris)
Recombinant
> 96 % by SDS-PAGE and HPLC analyses.
Active
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- Target See all Hirudin products
- Hirudin
- Protein Type
- Recombinant
- Biological Activity
- Active
- Origin
- Leech
- Source
- Yeast (Pichia pastoris)
- Sequence
- VVYTDCTESG QNLCLCEGSN VCGQGNKCIL GSDGEKNQCV TGEGTPGPQS HNDGDFEEPE EY
- Characteristics
- The biological activity is determined by chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific activity is no less than 10,000ATU/mg protein.
- Purity
- > 96 % by SDS-PAGE and HPLC analyses.
- Endotoxin Level
- Level Less than 10EU/mg of rhirudin as determined by LAL method
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
- Storage
- 4 °C
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- Target
- Hirudin
- Abstract
- Hirudin Products
- Background
- Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech Unlike heparin, hirudin act directly on thrombin, rather than through other clotting factors. They have a high binding affinity and specificity for thrombin. The mechanism of hirudin-thrombin binding appears to be unique. Recombinant hirudin variant is derived from yeast cell, the polypeptide containing 65 amino acid residues has a molecular weight of 6979.5 Da, which is identical to natural hirudin except for substitution of leucine for isoleucine at the N-terminal end of the molecule and the absence of a sulfate group on the tyrosine at position 63. Synonym: Hirudin. Formulation: Lyophilized from a 0.2µm filtered solution of 20mM PBS, pH 7.0, containing 2% mannitol.
- Molecular Weight
- Approximately 6.7k Da, containing 63 amino acid residues.
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