CCL16 Protein

Catalog No. ABIN987914

Product Details

Target: CCL16 (Chemokine (C-C Motif) Ligand 16 (CCL16))

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Sequence: QPKVPEWVNT PSTCCLKYYE KVLPRRLVVG YRKALNCHLP AIIFVTKRNR EVCTNPNDDW VQEYIKDPNL PLLPTRNLST VKIITAKNGQ PQLLNS

Characteristics: Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human monocytes is less than 100 ng/ml, corresponding to a specific activity of >, 1.0 × 104 IU/mg.

Purity: > 97 % by SDS-PAGE and HPLC analyses.

Endotoxin Level: Level Less than 1EU/µg of rHuHCC-4/CCL16 as determined by LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage: 4 °C

Target Details

Target: CCL16 (Chemokine (C-C Motif) Ligand 16 (CCL16))

Alternative Name: HCC-4/CCL16 (CCL16 Products)

Synonyms: CKb12 Protein, HCC-4 Protein, ILINCK Protein, LCC-1 Protein, LEC Protein, LMC Protein, Mtn-1 Protein, NCC-4 Protein, NCC4 Protein, SCYA16 Protein, SCYL4 Protein, C-C motif chemokine ligand 16 Protein, CCL16 Protein, Ccl16 Protein

Background: Human HCC-4, also named NCC-4, liver-expressed chemokine (LEC), and lymphocyte and monocyte chemoattractant (LMC), is a novel CC chemokine identified through bioinformatics. HCC-4 cDNA encodes a 120 amino acid (aa) residue precursor protein with a 23 aa residue predicted signal peptide that is cleaved to generate a 97 aa residue mature protein. HCC-4 is distantly related to other CC chemokines, exhibiting less than 30% sequence identity. Among these CC chemokines, HCC-4 has the most similarity to HCC-1. Two potential polyadenylation signals are present on the human HCC-4 gene, and as a result, two transcripts containing approximately 1,500 base pairs and 500 base pairs have been detected. HCC-4 is expressed weakly by some lymphocytes, including NK cells, T cells, and some T cell clones. The expression of HCC-4 in monocytes is highly upregulated in the presence of IL-10. Synonym: HCC-4/CCL16, Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.

Molecular Weight: 11.2 kDa, a single non-glycosylated polypeptide chain containing 97 amino acids.