CXCL2 Protein

Catalog No. ABIN987896

Product Details

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Sequence: APLATELRCQ CLQTLQGIHL KNIQSVKVKS PGPHCAQTEV IATLKNGQKA CLNPASPMVK KIIEKMLKNG KS

Characteristics: Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is less than 100 ng/ml, corresponding to a specific activity of >, 1.0 × 104 IU/mg.

Purity: > 97 % by SDS-PAGE and HPLC analyses.

Endotoxin Level: Level Less than 1EU/µg of rHuGRO-beta/CXCL2 as determined by LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage: 4 °C

Target Details

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Alternative Name: GRO-beta / CXCL2 (CXCL2 Products)

Synonyms: CINC-2a Protein, GRO2 Protein, GROb Protein, MGSA-b Protein, MIP-2a Protein, MIP2 Protein, MIP2A Protein, SCYB2 Protein, GRO3 Protein, Gro2 Protein, MIP-2 Protein, Mgsa-b Protein, Mip2 Protein, Scyb Protein, Scyb2 Protein, Mip-2 Protein, GROB Protein, MIP-2alpha Protein, C-X-C motif chemokine ligand 2 Protein, chemokine (C-X-C motif) ligand 2 Protein, CXCL2 Protein, Cxcl2 Protein

Background: The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively.Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B. Synonym: GRO-beta / CXCL2, Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 50mM NaCl.

Molecular Weight: 7.9 kDa, a single non-glycosylated polypeptide chain containing 73 amino acids.

Pathways: Cellular Response to Molecule of Bacterial Origin