Transferrin Protein (TF) (FITC)
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- Target See all Transferrin (TF) Proteins
- Transferrin (TF)
- Protein Type
- Native
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Origin
- Human
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Source
- Human
- Purification tag / Conjugate
- This Transferrin protein is labelled with FITC.
- Application
- Dot Blot (DB), Fluorescence Microscopy (FM)
- Supplier Product No.
- 009-0234
- Supplier
- Rockland
- Purpose
- Human Transferrin Fluorescein Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Human Transferrin and anti-Human Serum.
- Characteristics
- Human transferrin fluorescein conjugation, FITC conjugated transferrin
- Purification
- This product was prepared from normal serum by delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.
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- Discover our top product TF Protein
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- Application Notes
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Application Note: Human transferrin fluorescein conjugation has been tested in dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
IF Microscopy Dilution: 1:500-1:2500
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Transferrin (TF)
- Alternative Name
- Transferrin (TF Products)
- Background
- Background: Human transferrin is encoded by the TF gene and is an iron-binding blood plasma glycoprotein that controls the level of free iron in biological fluids. Human transferrin binds iron very tightly but reversibly. Human transferrin is the most important iron pool in mammals. Human transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of Human transferrin for Fe(III) is extremely high but decreases progressively with decreasing pH below neutrality.
- Gene ID
- 7018
- UniProt
- Q06AH7
- Pathways
- Transition Metal Ion Homeostasis
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