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Transferrin Protein (TF)

Rockland 009-0134 TF Origin: Human Host: Human Native WB, SDS, ELISA
Rockland
Catalog No. ABIN964544
Supplier Product No.: 009-0134
  • Target See all Transferrin (TF) Proteins
    Transferrin (TF)
    Protein Type
    Native
    Origin
    • 14
    • 7
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    Human
    Source
    • 15
    • 7
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA
    Supplier Product No.
    009-0134
    Supplier
    Rockland
    Purpose
    Human Transferrin- 009-0134
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Transferrin and anti-Human Serum.
    Characteristics
    apotransferrin, Transferrin Control, Siderophilin, TRF, apo-hTF
    Purification
    Human transferrin was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation followed by extensive dialysis. 
    Top Product
    Discover our top product TF Protein
  • Application Notes

    Application Note: Human Apo Transferrin from human serum was lyophilized from 0.2M ammonium bicarbonate for use in cell culture and assays. Transferrin has been tested in SDS-Page and is also suitable for use as antigen or ligand in immunochemical reactions, as a control or standard in assays, for conjugation and most other immunological methods requiring highly purified proteins.

    Western Blot Dilution: 1:2,000-1:10,000

    ELISA Dilution: 1:10,000 - 1:20,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Reconstitution Volume: 1.0 mL

    Concentration
    10.0 mg/mL
    Buffer

    Buffer: See application note.

    Stabilizer: None

    Preservative: None
    Preservative
    Without preservative
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    Transferrin (TF)
    Alternative Name
    Transferrin- (TF Products)
    Background
    Background: Human transferrin is encoded by the TF gene and is an iron-binding blood plasma glycoprotein that controls the level of free iron in biological fluids. Human transferrin binds iron very tightly but reversibly. Human transferrin is the most important iron pool in mammals. Human transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of Human transferrin for Fe(III) is extremely high but decreases progressively with decreasing pH below neutrality.
    Gene ID
    7018
    UniProt
    Q06AH7
    Pathways
    Transition Metal Ion Homeostasis
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