Streptavidin Protein (HRP)

Catalog No. ABIN964537

Product Details

Target: Streptavidin

Origin: Streptomyces avidinii

Host: Please inquire

Purification tag / Conjugate: This Streptavidin protein is labelled with HRP.

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB), Immunofluorescence (IF)

Purpose: Streptavidin Peroxidase Conjugated

Specificity: Streptavidin Peroxidase was prepared from chromatographically purified streptavidin. Streptavidin Peroxidase was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin. No reaction was observed against anti-Avidin.

Purification: Streptavidin-HRP was prepared from chromatographically purified streptavidin. Streptavidin Peroxidase conjugate was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin.

Components: Tested Applications: Dot Blot, WB
Suggested Applications: ELISA, EM, IF, IHC

Application Details

Application Notes: Streptavidin Peroxidase has been tested by dot blot and western blot and is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in Immunohistochemistry, Immunofluorescence, immuno-EM, Western Blotting, and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).

Comment:

Streptavidin Peroxidase is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Gentamicin Sulfate, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Handling Advice: Do NOT add Sodium Azide!

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Streptavidin Peroxidase conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Metzner, Luckert, Lemuth, Hämmerle, Moos: "Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection." in: Sensors (Basel, Switzerland), Vol. 18, Issue 5, (2018) (PubMed).

Vasek, Garber, Dorsey, Durrant, Bollman, Soung, Yu, Perez-Torres, Frouin, Wilton, Funk, DeMasters, Jiang, Bowen, Mennerick, Robinson, Garbow, Tyler, Suthar, Schmidt, Stevens, Klein: "A complement-microglial axis drives synapse loss during virus-induced memory impairment." in: Nature, Vol. 534, Issue 7608, pp. 538-43, (2016) (PubMed).

Cantoni, Bollman, Licastro, Xie, Mikesell, Schmidt, Yuede, Galimberti, Olivecrona, Klein, Cross, Otero, Piccio: "TREM2 regulates microglial cell activation in response to demyelination in vivo." in: Acta neuropathologica, Vol. 129, Issue 3, pp. 429-47, (2015) (PubMed).

Cowles, Li, Semmelhack, Cristea, Silhavy: "The free and bound forms of Lpp occupy distinct subcellular locations in Escherichia coli." in: Molecular microbiology, Vol. 79, Issue 5, pp. 1168-81, (2011) (PubMed).

Target Details

Target: Streptavidin

Abstract: Streptavidin Products

Background: Streptavidin is a a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditions that would typically cause dissociation (such as organic solvents, denaturants, detergents, and extreme temperatures or pH). Streptavidin's afinity for biotin can be employed in a variety of experimental uses, from purifications to standards, to means of detection or pull down experiments. Horse Raddish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).
Synonyms: HRP, Horseradish peroxidase, SA, S avidin

UniProt: P22629