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Protein G Protein (Alkaline Phosphatase (AP))

Origin: Streptococcus Recombinant ELISA
Catalog No. ABIN964531
  • Target See all Protein G Proteins
    Protein G
    Protein Type
    Recombinant
    Origin
    Streptococcus
    Host
    Please inquire
    Purification tag / Conjugate
    This Protein G protein is labelled with Alkaline Phosphatase (AP).
    Application
    ELISA
    Specificity
    Protein G Alkaline Phosphatase Conjugated was prepared from chromatographically pure Protein G. Protein G Alkaline Phosphatase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Protein G. No reaction was observed against anti-Protein A.
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  • Application Notes
    Protein G Alkaline Phosphatase Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader.
    Comment

    Protein G Alkaline Phosphatase Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Expiry Date
    12 months
  • Target
    Protein G
    Abstract
    Protein G Products
    Background
    Protein G is a surface protein of two groups of Streptococcal bacteria that has the ability to bind immunoglobulins. Similar to Protein A, but with slightly different specificity, Protein G is an important agent in the purification of proteins due to its ability to bind the Fc region. Protein G also binds to albumin. Alkaline Phosphatase is an enzyme which removes phosphate groups from a variety of substate molecules. As the name implies, this enzyme functions best under basic pH. Alkaline Phosphatase can be utilized in molecular biology in DNA ligation experiments (keeping the DNA linear), radiolabeling preparations, and a detection mediator in ELISA experiments. Protein G Alkalline Phosphatase Conjugated is ideal for investigators involved in Signal Transduction, Molecular Biology, Cancer, and Cell Signaling.
    Synonyms: ProG, Streptococcus G protein, ALP, Alk Phos
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