Protein A Protein (Sepharose)

Catalog No. ABIN964525

Product Details

Target: Protein A

Protein Type: Native

Origin: Staphylococcus aureus

Host: Please inquire

Purification tag / Conjugate: This Protein A protein is labelled with Sepharose.

Application: Immunoprecipitation (IP)

Characteristics: Concentration Definition: drained sepharose gel (~1 cc settled sepharose beads per 2 ml suspension)
Performance Data: Bead size 45-165 µm in size when swollen. ~35 mg Human IgG bound per cc of drained beads.

Application Details

Application Notes: Protein A resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.

Comment:

Sepharose 4 Fast Flow from GE/Amersham-Pharmacia

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 6.0 mg/cc

Buffer: 20% (v/v) Ethanol

Storage: 4 °C

Target Details

Target: Protein A

Abstract: Protein A Products

Synonyms: alpha protein Protein, repB Protein

Background: Sepharose™ Protein A is a suspension of beads conjugated to a fusion protein containing binding domains from protein A from Staphylococcus aureaus. When evenly dispersed approximately 2.0 cc of suspension is equal to 1.0 cc of settled beads of Sepharose™ 4 Fast Flow (highly cross linked, 4% agarose derivative). The bead size is approximately 45-165 µm when swollen. Approximately 6.0 mg of Protein A is bound per cc of drained beads. Each cc of drained beads will bind approximately 35 mg Human. The exclusion limit (Mr) of the beads is 3 x 10^7. The maximum linear flow rate is approximately 1300 cm/hr. This product shows long term stability within pH range of 3 to 9 and short term stability within pH range of 2 to 10.
Synonyms: Sepharose Protein A