gamma Globulin Fraction Protein

Catalog No. ABIN964153

Supplier Product No.: d612-0100

Product Details

Target: gamma Globulin Fraction

Protein Type: Native

Origin: Sheep

Source: Sheep

Application: SDS-PAGE (SDS), ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Supplier Product No.: d612-0100

Supplier: Rockland

Purpose: Sheep Gamma Globulin Fraction

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in precipitin arcs against anti-Sheep Serum corresponding to gamma globulins.

Characteristics: Plasma Gamma Globulin, Serum Gamma Globulin, Globulin Fractions, Gammaglobulin, sheep gamma fraction

Purification: Gamma Globulin was prepared from normal sheep serum by a multi-step process which includes delipidation and salt fractionation followed by extensive dialysis against the buffer stated above.

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Sheep Gamma Globulin Fraction has been tested by SDS-PAGE and can be utilized in Western Blotting and ELISA experiments.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Buffer: Restore with deionized water (or equivalent)

Reconstitution Volume: 10.0 mL

Concentration: 50 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Sheep Gamma at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: gamma Globulin Fraction

Background: Background: Sheep gamma globulin blocking reagent is an ideal blocker for western blotting, ELISA, Immunohistochemistry and other detection assays. Gamma globulins are a class of globulins, identified by their position after serum protein electrophoresis. The most significant gamma globulins are immunoglobulins ("Igs"), more commonly known as antibodies, although some Igs are not gamma globulins, and some gamma globulins are not Igs. Globulin is one of the three main types of serum proteins, the others being albumin and fibrinogen. Some globulins are produced in the liver, while others are made by the immune system.