gamma Globulin Fraction Protein

Catalog No. ABIN964144

Supplier Product No.: d604-1000

Product Details

Target: gamma Globulin Fraction

Protein Type: Native

Origin: Goat

Source: Goat

Application: SDS-PAGE (SDS), ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.: d604-1000

Supplier: Rockland

Purpose: Goat Gamma Globulin Fraction

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in precipitin arcs against anti-Goat Serum corresponding to gamma globulins.

Characteristics: Plasma Gamma Globulin, Serum Gamma Globulin, Globulin Fractions, Gammaglobulin, goat gamma fraction

Purification: Goat Gamma was prepared from normal goat serum by a multi-step process which includes delipidation and salt fractionation followed by extensive dialysis against the buffer stated above. 

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Goat Gamma Globulin Fraction has been tested by ELISA and SDS-PAGE and can be utilized in Western Blotting, ELISA experiments, and other detection assays.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Buffer: Restore with deionized water (or equivalent)

Reconstitution Volume: 100 mL

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store gamma globulin at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: gamma Globulin Fraction

Background: Background: Goat gamma globulin blocking reagent is an ideal blocker for western blotting, ELISA, Immunohistochemistry and other detection assays. Gamma globulins are a class of globulins, identified by their position after serum protein electrophoresis. The most significant gamma globulins are immunoglobulins ("Igs"), more commonly known as antibodies, although some Igs are not gamma globulins, and some gamma globulins are not Igs. Globulin is one of the three main types of serum proteins, the others being albumin and fibrinogen.