Biotin Protein (beta-Gal)
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- Target See all Biotin products
- Biotin
- Origin
- Chemical
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Source
- Synthetic
- Purification tag / Conjugate
- This Biotin protein is labelled with beta-Gal.
- Application
- ELISA
- Specificity
- Biotin Beta Galactosidase Conjugated has been assayed against 1.0 μg of Streptavidin in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1, 000 to 1:3, 000 is suggested for Biotin Beta Galactosidase Conjugated. Biotin Beta Galactosidase Conjugated has been assayed against 1.0 μg of Streptavidin in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1, 000 is suggested for Biotin Beta Galactosidase Conjugated.
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Biotin protein (Alkaline Phosphatase (AP))
Origin: Chemical Host: Synthetic
Biotin protein (Glucose Oxidase)Origin: Chemical Host: Synthetic ELISA, WB
Biotin protein (HRP)Origin: Chemical Host: Synthetic ELISA, WB
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- Application Notes
- Biotin Beta Galactosidase Conjugated has been assayed against 1.0 ug of Streptavidin in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 is suggested for Biotin Beta Galactosidase Conjugated. Biotin Beta Galactosidase Conjugated has been assayed against 1.0 ug of Streptavidin in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 is suggested for Biotin Beta Galactosidase Conjugated.
- Comment
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Biotin Beta Galactosidase Conjugated has been assayed against 1.0 ug of Streptavidin in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1, 000 to 1:3, 000 is suggested for Biotin Beta Galactosidase Conjugated. Biotin Beta Galactosidase Conjugated has been assayed against 1.0 ug of Streptavidin in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1, 000 is suggested for Biotin Beta Galactosidase Conjugated.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 0.05 M Potassium Phosphate, 0.001 M Magnesium Chloride, 0.1 M Beta-Mercaptoethanol, pH 7.8
- Storage
- 4 °C
- Expiry Date
- 12 months
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- Target
- Biotin
- Abstract
- Biotin Products
- Target Type
- Chemical
- Background
- Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin
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