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Avidin Protein (AVD) (Glucose Oxidase)

AVD Origin: Chicken egg ELISA, WB
Catalog No. ABIN964122
  • Target See all Avidin (AVD) Proteins
    Avidin (AVD)
    Origin
    • 11
    • 7
    Chicken egg
    Host
    Please inquire
    Purification tag / Conjugate
    This Avidin protein is labelled with Glucose Oxidase.
    Application
    ELISA, Western Blotting (WB)
    Specificity
    Avidin Glucose Oxidase was prepared from chromatographically pure avidin followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin and anti-Glucose Oxidase.
    Sterility
    Sterile filtered
    Top Product
    Discover our top product AVD Protein
  • Application Notes
    Avidin Glucose Oxidase Conjugated is a useful detection reagent for primary antibodies conjugated to biotin. Avidin Glucose Oxidase Conjugated can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).
    Comment

    Avidin Glucose Oxidase Conjugated is a useful detection reagent for primary antibodies conjugated to biotin. Avidin Glucose Oxidase Conjugated can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
    Concentration
    2.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Expiry Date
    12 months
  • Target
    Avidin (AVD)
    Alternative Name
    Avidin (AVD Products)
    Synonyms
    avidin Protein, avd Protein, LOC723980 Protein, AVD Protein
    Background
    Avidin is a biotin-binding protein found in the oviducts of egg-laying animals (birds, reptiles, and frogs) that gets deposited into the whites of their eggs. Avidin is a tetramer and can bind up to four biotin molecules (Vitamin B7) with one of the greatest known non-covalent interactions. Avidity for biotin is destroyed with heat. Glucose oxidase is an enzyme that drives the oxidation of glucose into hydrogen peroxide and D-glucono-δ-lactone. This activity is utilized in biotechnology through colormetric detection assays that are sensitive to the amount of hydrogen peroxide produced. Avidin Peroxidase Conjugated is ideal for investigators in Immunology, Cancer, Neuroscience, and Cell Biology.
    Synonyms: AvD, Avidin Biotin Complex, GOD, GOx
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