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- Target See all BSA products
- BSA (Bovine Serum Albumin (BSA))
- Protein Type
- Native
- Origin
- Cow
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Source
- Cow
- Purification tag / Conjugate
- This BSA protein is labelled with Texas Red (TR).
- Application
- Dot Blot (DB)
- Supplier Product No.
- 001-0933
- Supplier
- Rockland
- Purpose
- Bovine Albumin Texas Red™ Conjugated (BSA)
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Serum.
- Characteristics
- Bovine Albumin Texas Red™ conjugation, BSA Texas Red™ conjugation, Texas Red™ conjugated BSA , Texas Red™ conjugated Bovine Albumin
- Purification
- This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and selective precipitation followed by extensive dialysis against the buffer stated above.
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- Application Notes
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Application Note: BOVINE ALBUMIN Texas Red™ conjugated (BSA) has been tested in dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, and utilizing various commercial platforms.
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- BSA (Bovine Serum Albumin (BSA))
- Alternative Name
- Bovine Albumin (BSA Products)
- Background
- Background: Bovine Serum Albumin (BSA) is used for various biochemical applications including ELISA (Enzyme-Linked Immunosorbent Assay), high content screening assays, western blotting, FACS Buffer and immunohistochemistry. BSA as a blocking reagent is particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Bovine Serum Albumin can also be used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA.
- Pathways
- Lipid Metabolism
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