ALOX5 Protein
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- Target See all ALOX5 Proteins
- ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Baculovirus
- Characteristics
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Purified recombinant Human 5-Lipoxygenase protein
Expression System: Baculovirus
Bioactivity: 20 units/mg protein - One unit is defined as the amount of enzyme that consumes 1.0 nmol of oxygen per min at 25 °C in 50 mM Tris-HCl, 100 uM arachidonate, 2 mM CaCl2, 1 mM ATP, 250 uM oxygen. Oxygen consumption can be measured using a oxygraph equipped with a Clark oxygen electrode. - Top Product
- Discover our top product ALOX5 Protein
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- Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
- Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris containing 0 mM EDTA, pH 8.0.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- -80 °C
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- Target
- ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))
- Alternative Name
- 5-Lipoxygenase (ALOX5 Products)
- Background
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5-lipoxygenase (5-LO) catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A4. It is localized in the cytosol of resting human and rat peripheral blood neutrophils, whereas it is found in the nucleus in rat basophilic leukemia cells and human alveolar macrophages. Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA2.
Alternative Names: 5-Lipoxygenase Antigen, 5 Lipoxygenase Recombinant Protein - Molecular Weight
- 78 kDa
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