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FKBP3 Protein (AA 1-224)

FKBP3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % pure SDS Active
Catalog No. ABIN935093
  • Target See all FKBP3 Proteins
    FKBP3 (FK506 Binding Protein 3, 25kDa (FKBP3))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 1-224
    Origin
    • 8
    • 2
    • 2
    • 1
    • 1
    Human
    Source
    • 9
    • 3
    • 1
    • 1
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS)
    Sequence
    MAAAVPQRAW TVEQLRSEQL PKKDIIKFLQ EHGSDSFLAE HKLLGNIKNV AKTANKDHLV TAYNHLFETK RFKGTESISK VSEQVKNVKL NEDKPKETKS EETLDEGPPK YTKSVLKKGD KTNFPKKGDV VHCWYTGTLQ DGTVFDTNIQ TSAKKKKNAK PLSFKVGVGK VIRGWDEALL TMSKGEKARL EIEPEWAYGK KGQPDAKIPP NAKLTFEVEL VDID
    Characteristics
    Purified recombinant Human FKBP3 protein
    Expression System: E.coli
    Bioactivity: Specific activity is > 120 nM/min/µg, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH 8.0 using chymotrypsin.
    Purity
    > 90 % pure
    Top Product
    Discover our top product FKBP3 Protein
  • Application Notes
    FKBP3 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
    Assay Procedure
    1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
      2. Add recombinant FKBP3 protein with various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
      3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer.
      4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
      5. Record the increase in A410 nm for 20 minutes
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT and 10 % glycerol.
    Preservative
    Dithiothreitol (DTT)
    Precaution of Use
    This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze/thaw cycles.
    Storage
    RT/-20 °C
    Storage Comment
    Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
  • Target
    FKBP3 (FK506 Binding Protein 3, 25kDa (FKBP3))
    Alternative Name
    FKBP3 (FKBP3 Products)
    Synonyms
    FKBP3 Protein, 25kDa Protein, FKBP-3 Protein, FKBP25 Protein, FK506 Protein, fb75c04 Protein, si:dz261o22.2 Protein, wu:fb75c04 Protein, zgc:110728 Protein, FKBP-25 Protein, PPIase Protein, FK506 binding protein 3 Protein, FK506 binding protein 3 L homeolog Protein, peptidylprolyl isomerase Protein, FKBP3 Protein, Fkbp3 Protein, fkbp3 Protein, fkbp3.L Protein, CAALFM_C103790CA Protein
    Background
    FK506 binding protein 3 (FKBP3), also known as FKBP25, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP3 associates with transcriptional repressor protein YY1 and histone deaceltylases, HDAC1 and HDAC2. Also, FKBP3 may contain several casein kinase II phosphorylation sites, which are believed to be important for cell growth regulation. It is localized in the nucleus and is expressed in the brain, testis, ovary, and spleen. Recombinant human FKBP3 was expressed in E. coli and purified by using conventional chromatography techniques.
    Alternative Names: PPIase protein, FK506 binding protein 3 FKBP 25 protein, FKBP-3 protein, FKBP3, PPIase FKBP3 protein, FKBP-3, Rotamase protein, Rotamase., FKBP25 protein, FKBP 3, FKBP 3 protein, FKBP 3 protein
    Molecular Weight
    25.1 kDa (224 AA)
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