ENO1 Protein (AA 1-434)

Catalog No. ABIN935064

Product Details

Target: ENO1 (Enolase 1 (ENO1))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 1-434

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Sequence: MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK

Characteristics: Purified recombinant Human Alphaenolase protein
Expression System: E.coli
Bioactivity: Specific activity: > 13 units/mL. One unit will convert 1.0 m of 2-phosphoglycerate to phospho(enol)pyruvate per minute at pH 7.5 at 25C

Purity: > 90 % pure

Application Details

Application Notes: Alphaenolase protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris-HCl buffer, pH 7.5, containing 1 mM MgSO4 and 10 % glycerol.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: ENO1 (Enolase 1 (ENO1))

Abstract: ENO1 Products

Synonyms: enolase 1 Protein, F2P9.10 Protein, F2P9_10 Protein, Pgh1 Protein, pENO1 Protein, enolase 1 Protein, phosphopyruvate hydratase Protein, ENO1 Protein, eno1 Protein

Background: Alpha-enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of alpha-enolase is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoprotein associated with Hashimoto's encephalopathy and severe asthma. Recombinant human alpha-enolase was expressed in E. coli and purified by using conventional chromatography.
Alternative Names: Enolase 1 protein, C myc promoter binding protein protein, 2 phospho D glycerate hydro lyase protein, Alpha enolase protein, Phosphopyruvate hydratase protein, MPB 1 protein, ENO1L1 protein, MYC promoter-binding protein 1 protein, Non neural enolase protein, Enolase 1 (alpha) like 1 protein, Non-Neuronal Enolase (NNE) protein, MYC promoter binding protein 1 protein, EC 4.2.1.11 protein, NNE protein, MBP-1 protein, PPH protein, Enolase-1 protein, , MBPB1 protein, PPH protein, Plasminogen binding protein protein, MBP 1 protein, ENO1 protein, Enolase 1 (alpha) protein, tau-crystallin protein, MPB1 protein, MBP1 protein

Molecular Weight: 47.1 kDa (434 AA)