VPS28 Protein (AA 1-221)
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- Target See all VPS28 Proteins
- VPS28 (Vacuolar Protein Sorting 28 (VPS28))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-221
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Sequence
- MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ ALEKAYIKDC VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR LDCPLAMERI KEDRPITIKD DKGNLNRCIA DVVSLFITVM DKLRLEIRAM DEIQPDLREL METMHRMSHL PPDFEGRQTV SQWLQTLSGM SASDELDDSQ VRQMLFDLES AYNAFNRFLH A
- Characteristics
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Purified recombinant Human VPS28 protein
Expression System: E.coli - Purity
- > 95 % pure
- Endotoxin Level
- < 1.0 EU per µg of protein (determined by LAL method )
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Vacuolar Protein Sorting 28 (VPS28) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)
VPS28 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Vacuolar Protein Sorting 28 (VPS28) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)VPS28 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- VPS28 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Supplied as a liquid in 20 mM Tris-HCl buffer, pH 7.5, containing 0 mM DTT and 10 % glycerol.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- RT/-20 °C
- Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- Target
- VPS28 (Vacuolar Protein Sorting 28 (VPS28))
- Alternative Name
- VPS28 (VPS28 Products)
- Background
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VPS28 is involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. This protein is a subunit of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. VSP28 binding with the TSG101 may play important roles in budding of Retroviruses, such as HIV-1 and Ebola virus. Recombinant human VPS28 was expressed in E. coli and purified by using conventional chromatography.
Alternative Names: VPS 28 protein, VPS-28, Vacuolar Protein Sorting 28 acuolar protein sorting 28 protein, VPS 28, VPS28, ESCRT-I complex subunit VPS28 protein, Vacuolar protein sorting 28 (yeast) protein, H-Vps28 protein, VPS-28 protein, Vacuolar protein sorting 28 homolog. - Molecular Weight
- 25.4 kDa (221 AA)
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