PCNA Protein (AA 1-261)
PCNA
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 95 % pure
SDS
1 image
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- Target See all PCNA Proteins
- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-261
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Sequence
- MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQL TLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN EPVQLTFALR YLNFFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S
- Characteristics
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Purified recombinant Human PCNA protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher. - Purity
- > 95 % pure
- Top Product
- Discover our top product PCNA Protein
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Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) protein (GST tag)
PCNA Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Proliferating Cell Nuclear Antigen (PCNA) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PCNA Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- PCNA protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Supplied as a liquid in 20 mM Tris, pH 7.5, containing 0 mM EDTA, and 20 % glycerol.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- RT/-20 °C
- Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- Target
- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
- Alternative Name
- PCNA (PCNA Products)
- Background
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PCNA (Proliferating Cell Nuclear protein) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by UV irradiation, growth factors and eventually by neighbouring tumours. Recombinant human PCNA, was expressed in E. coli and purified by conventional chromatography techniques.
Alternative Names: PCNA protein, , Proliferating cell nuclear protein protein, MGC8367 protein, Proliferating cell nuclear protein protein - Molecular Weight
- 28 kDa (261 AA)
- Pathways
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
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