HSV1 gD Protein (AA 21-339) (His tag)

Catalog No. ABIN810025

Product Details

Target: HSV1 gD (Herpes Simplex Virus Type 1, Glycoprotein D (HSV1 gD))

Protein Type: Recombinant

Protein Characteristics: AA 21-339

Origin: Herpes Simplex Virus (HSV)

Source: Yeast (Pichia pastoris)

Purification tag / Conjugate: This HSV1 gD protein is labelled with His tag.

Application: ELISA, Western Blotting (WB)

Characteristics: Recomb. Recombinant Herpes Simplex Virus-1 gD (HSV-1) glycosylated. Glycosylated recombinant ecto-domain of HSV-1 gD (AA 21-339 from KOS strain) representing the external domain of the gD molecule without membrane spanning and cytosolic sequences. It encodes a protein of approximately 30 kDa that contains 3 sites for glycosylation. Contains a 6-histidine fusion partner. The yeast system adds a mannose core oligosaccharide to N-linked motifs. Glycosylation is heterogeneous and results in several immunoreactive products greater than 40 kDa on Western blots. The doublet band at approximately 28 and 30 kDa is not believed to be glycosylated.

Purification: Purity verified by SDS-PAGE. Purity compares with reference lot.

Application Details

Application Notes: Suitable for use in ELISA and Western blot. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Comment:

Infectious Disease Antigens and Toxins

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Sodium phosphate, 0.00 - 0.1 M Sodium chloride, pH 6.7 to 7.7

Preservative: Without preservative

Handling Advice: Avoid multiple freeze/thaw cycles.

Storage: -80 °C

Storage Comment: Store at -70 °C or below.

Target Details

Target: HSV1 gD (Herpes Simplex Virus Type 1, Glycoprotein D (HSV1 gD))

Abstract: HSV1 gD Products

Target Type: Viral Protein

Molecular Weight: 30 kDa