HIV-2 gp36 protein (His tag)
Origin: Human
Host: Yeast (Pichia pastoris)
Recombinant
ELISA, WB
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- Target
- HIV-2 gp36
- Protein Type
- Recombinant
- Origin
- Human
-
Source
- Yeast (Pichia pastoris)
- Purification tag / Conjugate
- His tag
- Application
- ELISA, Western Blotting (WB)
- Characteristics
- Human Immunodeficiency Virus Type 2 (HIV-2) gp36, Recombinant ecto-domain of HIV-2 gp36. Contains a 6 histidine fusion partner. Reacts with HIV-2 positive human sera and monoclonal antibodies as determined by ELISA and Western blot. Major immunospecific band at 14 kDa, minor bands at 5, 16, 18, 28 and 42 kDa.
- Purification
- Purity determined by SDS-PAGE and Western blot
-
HIV-2 gp36 protein (His tag,MBP tag)
Origin: Human Immunodeficiency Virus (HIV) Host: Escherichia coli (E. coli) Recombinant > 90 % as determined by SDS-PAGE
-
- Application Notes
- Suitable for use in ELISA and Western blot. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. HIV-2 gp36 ELISA with HIV-2 positive sera at 1:2.000 with 1-100 nanograms per well gp36. Background with BSA or normal sera OD405 <0.1. Alkaline phosphatase substrate for 45 min.
- Comment
-
Infectious Disease Antigens and Toxins
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- 6 M Urea, 0.02 M Tris-HCl, 0.5 M Sodium chloride, pH 7.0 to 8.0 at room temperature
- Preservative
- Without preservative
- Handling Advice
- Aliquot to avoid multiple freeze thaws.
- Storage
- -80 °C
- Storage Comment
- Store at -70 °C or below.
-
- Target
- HIV-2 gp36
- Target Type
- Viral Protein
- Molecular Weight
- 14 kDa
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