E2F3 Protein (AA 1-457) (His tag)
custom-made
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all E2F3 Proteins
- E2F3
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-457
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This E2F3 protein is labelled with His tag.
- Purpose
- Custom-made recombinant E2f3 Protein expressed in mammalian cells.
- Sequence
- MRKGIQPALE QYLVTAGGGE GAAVVAAAAA ASMDKRALLA SPGFAAAAAP GTYIQILTTN PSTTSCATSL QSGALTAGPL LPSVPGTEPA ASSLYTTPQG PSSRVGLLQQ PPAPGRGGGG GPPAKRRLEL GESGHQYLSD GLKTPKGKGR AALRSPDSPK TPKSPSEKTR YDTSLGLLTK KFIQLLSQSP DGVLDLNKAA EVLKVQKRRI YDITNVLEGI HLIKKKSKNN VQWMGCSLSE DGGMLAQCQG LSKEVTELSQ EEKKLDELIQ SCTLDLKLLT EDSENQRLAY VTYQDIRKIS GLKDQTVIVV KAPPETRLEV PDSIESLQIH LASTQGPIEV YLCPEETETH RPMKTNNQDH NGNIPKPTSK DLASNNSGHS DCSVSTANLS PLASPANLLQ QTEDQIPSNL EGPFVNLLPP LLQEDYLLSL GEEEGISDLF DAYDLEKLPL VEDFMCS Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
- Top Product
- Discover our top product E2F3 Protein
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E2F3 (AA 1-133) protein (GST tag)
Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- E2F3
- Abstract
- E2F3 Products
- Background
- Transcription factor E2F3 (E2F-3),FUNCTION: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters (PubMed:20176812). {ECO:0000269|PubMed:20176812}.
- Molecular Weight
- 48.8 kDa
- UniProt
- O35261
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