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E2F3 Protein (AA 1-465) (His tag)

custom-made Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Catalog No. ABIN7547252
  • Target See all E2F3 Proteins
    E2F3
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-465
    Origin
    • 3
    • 3
    Human
    Source
    • 2
    • 2
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This E2F3 protein is labelled with His tag.
    Purpose
    Custom-made recombinant E2F3 Protein expressed in mammalian cells.
    Sequence
    MRKGIQPALE QYLVTAGGGE GAAVVAAAAA ASMDKRALLA SPGFAAAAAA AAAPGAYIQI LTTNTSTTSC SSSLQSGAVA AGPLLPSAPG AEQTAGSLLY TTPHGPSSRA GLLQQPPALG RGGSGGGGGP PAKRRLELGE SGHQYLSDGL KTPKGKGRAA LRSPDSPKTP KSPSEKTRYD TSLGLLTKKF IQLLSQSPDG VLDLNKAAEV LKVQKRRIYD ITNVLEGIHL IKKKSKNNVQ WMGCSLSEDG GMLAQCQGLS KEVTELSQEE KKLDELIQSC TLDLKLLTED SENQRLAYVT YQDIRKISGL KDQTVIVVKA PPETRLEVPD SIESLQIHLA STQGPIEVYL CPEETETHSP MKTNNQDHNG NIPKPASKDL ASTNSGHSDC SVSMGNLSPL ASPANLLQQT EDQIPSNLEG PFVNLLPPLL QEDYLLSLGE EEGISDLFDA YDLEKLPLVE DFMCS Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Specificity
    If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
    Characteristics
    Key Benefits:
    • Made to order protein - from design to production - by highly experienced protein experts.
    • Protein expressed in mammalian cells and purified in one-step affinity chromatography
    • The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).
    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    If you are not interested in a full length protein, please contact us for individual protein fragments.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
    Purity
    > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
    Grade
    custom-made
    Top Product
    Discover our top product E2F3 Protein
  • Application Notes
    We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    12 months
  • Target
    E2F3
    Abstract
    E2F3 Products
    Background
    Transcription factor E2F3 (E2F-3),FUNCTION: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters (By similarity). {ECO:0000250|UniProtKB:O35261}.
    Molecular Weight
    49.2 kDa
    UniProt
    O00716
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