WNT5A Protein (His tag)

Catalog No. ABIN7539361

Product Details

Target: WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This WNT5A protein is labelled with His tag.

Purpose: Wnt-5a

Sequence: MIIGAQPLCS QLAGLSQGQK KLCHLYQDHM QYIGEGAKTG IKECQYQFRH RRWNCSTVDN TSVFGRVMQI GSRETAFTYA VSAAGVVNAM SRACREGELS TCGCSRAARP KDLPRDWLWG GCGDNIDYGY RFAKEFVDAR ERERIHAKGS YESARILMNL HNNEAGRRTV YNLADVACKC HGVSGSCSLK TCWLQLADFR KVGDALKEKY DSAAAMRLNS RGKLVQVNSR FNSPTTQDLV YIDPSPDYCV RNESTGSLGT QGRLCNKTSE GMDGCELMCC GRGYDQFKTV QTERCHCKFH WCCYVKCKKC TEIVDQFVCK LEHHHHHH

Characteristics: Length (aa):328

Purity: > 90 % by SDS-PAGE and Coomassie stain

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Mouse Wnt5a should be reconstituted in water to a concentration of 0.1 mg/mL. This solution can be diluted in water or other buffer solutions or stored at -20 °C.

Buffer: 50 mM acetic acid

Storage: RT,0 °C,-20 °C

Storage Comment: The lyophilized mouse Wnt5a, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted human Wnt5a should be stored in working aliquots at -20°C.

Target Details

Target: WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))

Alternative Name: Wnt-5a (WNT5A Products)

Synonyms: hWNT5A Protein, wnt-5A Protein, wnt5a-B Protein, WNT5A Protein, 8030457G12Rik Protein, Wnt-5a Protein, Wnt family member 5A Protein, Wnt family member 5A S homeolog Protein, wingless-type MMTV integration site family, member 5a Protein, wingless-type MMTV integration site family, member 5A Protein, wingless-related MMTV integration site 5A Protein, Wnt signaling ligand Protein, WNT5A Protein, Wnt5a Protein, wnt5a.S Protein, wnt5a Protein, LOC578814 Protein, wnt5 Protein

Background: Wnt-5a, Wnt5a, Wnt 5a, Wnt,Wnt-5a, a member of the highly conserved Wnt protein family, is a 352 amino acid protein containing 23 conserved cysteines and contains a cysteine modified by palmitate which is essential for receptor binding and biological activity. Wnt-5a is one of the most highly investigated non-canonical Wnts and has been implicated in almost all aspects of non-canonical Wnt signaling. In terms of cancer development, Wnt-5a has, until recently, lived in the shadow of its better-characterized relatives. This was largely because of its apparent inability to transform cells or signal through the canonical beta-catenin pathway that is so important in cancer, particularly colorectal cancer. Recent work in a wide range of human tumours has pointed to a critical role for Wnt-5a in malignant progression, but there is conflicting evidence whether Wnt-5a has a tumour-promoting or -suppressing role. Emerging evidence suggests that the functions of Wnt-5a can be drastically altered depending on the availability of key receptors. Hence, the presence or absence of these receptors may go some way to explain the conflicting role of Wnt-5a in different cancers.

Molecular Weight: 36.9 kDa

Gene ID: 22418

NCBI Accession: NM_009524

UniProt: P22725

Pathways: WNT Signaling, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size, Tube Formation