MPXV A35R (AA 59-181) protein (His tag)
Origin: Mpox Virus
Host: HEK-293 Cells
Recombinant
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining,>95 %
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- Target
- MPXV A35R
- Protein Type
- Recombinant
- Protein Characteristics
- AA 59-181
- Origin
- Mpox Virus
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- His tag
- Purpose
- Recombinant Mpox (formerly known as monkeypox) virus EEV envelope glycoprotein (A35R) with a C-terminal His-tag, derived from the transfected HEK293 cells.
- Sequence
- Gln59-Thr181
- Purification
- His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
- Purity
- SDS-PAGE under reducing conditions and visualized by Coomassie blue staining,>95 %
- Endotoxin Level
- <0.5 EU per μg of the protein as determined by the LAL method
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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Filtered solution in PBS with 1 % mannitol and 5 % trehalose
- Storage
- -20 °C
- Storage Comment
- -20°C
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- Target
- MPXV A35R
- Molecular Weight
- Recombinant protein product has a calculated molecular mass of ~14 kDa. Due to the abundant glycosylation, it migrates as one major ~14 kDa protein band (non-glycosylated form) and slight increased smear bands (glycosylated form) in SDS-PAGE under DTT and
- UniProt
- Q8V4U4
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