MPXV A30L (AA 22-146) protein (His tag)
Origin: Mpox Virus
Host: HEK-293 Cells
Recombinant
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
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- Target
- MPXV A30L
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-146
- Origin
- Mpox Virus
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- His tag
- Purpose
- Recombinant Mpox (formerly known as monkeypox) virus (MPXV) envelope protein A28 homolog with C-terminal His-tag, derived from the transfected human HEK293 cells.
- Sequence
- Gln22-Leu146
- Purification
- His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
- Purity
- SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
- Endotoxin Level
- <0.5 EU per μg of the protein as determined by the LAL method
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.
- Buffer
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Lyophilized from a 0.2 μm filtered solution in PBS ( pH 7.4) with 1 % mannitol and 5 % trehalose
- Storage
- -20 °C
- Storage Comment
- -20°C
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- Target
- MPXV A30L
- Molecular Weight
- Recombinant protein product has a calculated molecular mass of ~14 kDa. Due to the abundant glycosylation, it migrates as approximately ~20-30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
- UniProt
- Q8V4U9
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