SLC1A2 Protein (YFP tag)

Catalog No. ABIN7538793

Product Details

Target: SLC1A2 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 2 (SLC1A2))

Protein Type: cVLP

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This SLC1A2 protein is labelled with YFP tag.

Application: Western Blotting (WB), ELISA, Nanoparticle Tracking Analysis (NTA)

Purpose: Chimeric VLP of SARS-CoV-2 (M+E+N) EAAT2-EYFP

Sequence: AA 1 - 506 (c-terminal truncation )

Characteristics: Chimeric SARS-CoV-2 virus-like particles (cVLP) were produced in HEK cells by co-expression of the membrane-, envelope- and nucleoprotein with a membrane bound target protein. The VLPs do not contain the viral genome, cannot replicate and are not infectious.

Purification: Polyethylene glycol precipitation

Biological Activity Comment: active

Application Details

Application Notes: Applications: Immunogenic antigen, antigen for ELISA and Western blot.
Western blot: 1-10 μg, ELISA: 1-5 μg/mL

Comment:

Virus-like Particles are multiprotein complexes that resemble a native virus, but lack the genetic information. Therefore, VLPs are safe to handle in numerous fields of applications. For example, VLPs can be applied as antigen in serological assays (e.g. ELISA), or serve as reference material to standardize the performance of different diagnostic tests (e.g. rapid antigen tests or ELISA). Due to the self-adjuvanting properties of VLPs is the most common application of VLPs the use as antigen for immunizations for vaccine development or antibody discovery campaigns.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS

Storage: -80 °C

Storage Comment: - 80°C

Target Details

Target: SLC1A2 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 2 (SLC1A2))

Alternative Name: Excitatory amino acid transporter 2 (SLC1A2 Products)

Synonyms: CG3159 Protein, Dmel\\CG3159 Protein, EAAT Protein, dEAAT2 Protein, dEaat2 Protein, Eaat Protein, GB16377 Protein, EAAT2 Protein, SLC1A2 Protein, Eaat2 Protein, Glt Protein, Glt-1 Protein, GLT-1 Protein, 1700091C19Rik Protein, 2900019G14Rik Protein, AI159670 Protein, GLT1 Protein, MGLT1 Protein, eaat2 Protein, fj34b12 Protein, slc1a2 Protein, wu:fj34b12 Protein, zgc:65897 Protein, Excitatory amino acid transporter 2 Protein, excitatory amino acid transporter 2 Protein, solute carrier family 1 member 2 Protein, solute carrier family 1 (glial high affinity glutamate transporter), member 2 Protein, solute carrier family 1 (glial high affinity glutamate transporter), member 2b Protein, Eaat2 Protein, Eaat-2 Protein, SLC1A2 Protein, VDBG_06180 Protein, EUBELI_01747 Protein, Trebr_0139 Protein, Slc1a2 Protein, slc1a2b Protein

Background: Excitatory amino acid transporter 2 (EAAT2), also known as solute carrier family 1 member 2 (SLC1A2) and glutamate transporter 1 (GLT-1), is encoded by the SLC1A2 gene in humans. The membrane-bound protein is the main transporter that removes the excitatory neurotransmitter glutamate from the extracellular space at the synapses in the central nervous system. Dysregulation of EAAT2 is associated with neurodegenerative diseases and excitotoxicity. SARS-CoV-2 (Severe acute respiratory syndrome coronavirus type 2) is a coronavirus (genus: Betacoronavirus, subgenus: Sarbecovirus) that was identified as the cause of COVID-19 disease in early 2020.

Pathways: Dicarboxylic Acid Transport