TLR5 Protein

Catalog No. ABIN7538553

Product Details

Target: TLR5 (Toll-Like Receptor 5 (TLR5))

Protein Type: Synthetic Nanodisc

Origin: Human

Source: Mammalian Cells

Purpose: Human TLR5 full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Comment:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: TLR5 (Toll-Like Receptor 5 (TLR5))

Alternative Name: TLR5 (TLR5 Products)

Synonyms: SLEB1 Protein, TIL3 Protein, TLR-5 Protein, sleb1 Protein, til3 Protein, tlr-5 Protein, tlr5 Protein, toll like receptor 5 Protein, toll-like receptor 5 Protein, toll like receptor 5 L homeolog Protein, toll-like receptor 5b Protein, TLR5 Protein, Tlr5 Protein, tlr5.L Protein, tlr-5 Protein, tlr5b Protein

Background: Toll-like receptor (TLR) family plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.

Molecular Weight: The human full length TLR5 protein has a MW of 97.8 kDa

UniProt: O60602

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades