Serotonin Receptor 1E Protein (HTR1E)

Catalog No. ABIN7538521

Product Details

Target: Serotonin Receptor 1E (HTR1E)

Protein Type: Synthetic Nanodisc

Origin: Human

Source: Mammalian Cells

Purpose: Human 5HT1E full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Comment:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Serotonin Receptor 1E (HTR1E)

Alternative Name: 5HT1E (HTR1E Products)

Synonyms: Htr1ea Protein, HTR1E Protein, 5HT1E Protein, 5-HT1E Protein, 5-HT-1E Protein, 5-hydroxytryptamine (serotonin) receptor 1E Protein, 5-hydroxytryptamine receptor 1E Protein, 5-hydroxytryptamine (serotonin) receptor 1E, G protein-coupled Protein, Htr1e Protein, HTR1E Protein, htr1e Protein

Background: G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.[UniProtKB/Swiss-Prot Function]

Molecular Weight: The human full length 5HT1E protein has a MW of 41.7kDa

UniProt: P28566

Pathways: JAK-STAT Signaling, cAMP Metabolic Process