PTGER3 Protein

Catalog No. ABIN7538491

Product Details

Target: PTGER3 (Prostaglandin E Receptor 3 (Subtype EP3) (PTGER3))

Protein Type: Synthetic Nanodisc

Origin: Human

Source: Mammalian Cells

Purpose: Human PE2R3 full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Comment:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: PTGER3 (Prostaglandin E Receptor 3 (Subtype EP3) (PTGER3))

Alternative Name: PE2R3 (PTGER3 Products)

Synonyms: PTGER3 Protein, EP3 Protein, EP3-I Protein, EP3-II Protein, EP3-III Protein, EP3-IV Protein, EP3e Protein, PGE2-R Protein, PTGEREP3 Protein, Rep3 Protein, rEP3a Protein, rEP3b Protein, Pgerep3 Protein, Ptgerep3 Protein, prostaglandin E receptor 3 Protein, prostaglandin E receptor 3 L homeolog Protein, prostaglandin E receptor 3 (subtype EP3) Protein, PTGER3 Protein, ptger3.L Protein, Ptger3 Protein

Background: The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor may have many biological functions, which involve digestion, nervous system, kidney reabsorption, and uterine contraction activities. Studies of the mouse counterpart suggest that this receptor may also mediate adrenocorticotropic hormone response as well as fever generation in response to exogenous and endogenous stimuli. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

Molecular Weight: The human full length PE2R3 protein has a MW of 43.3kDa

UniProt: P43115