GPR109B Protein
HCAR3
Origin: Human
Host: Mammalian Cells
Synthetic Nanodisc
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- Target See all GPR109B (HCAR3) products
- GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))
- Protein Type
- Synthetic Nanodisc
- Origin
- Human
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Source
- Mammalian Cells
- Purpose
- Human HCAR3 full length protein-synthetic nanodisc
- Characteristics
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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Hydroxycarboxylic Acid Receptor 3 (HCAR3) (AA 1-387) protein (GST tag)
HCAR3 Origin: Human Host: Wheat germ Recombinant WB, AP, ELISA, AA
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- Comment
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Advantages of Synthetic Nanodiscs:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- Expiry Date
- 12 months
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- Target
- GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))
- Alternative Name
- HCAR3 (HCAR3 Products)
- Synonyms
- GPR109B Protein, HCA3 Protein, HM74 Protein, PUMAG Protein, Puma-g Protein, NIACR2 Protein, hm74 Protein, niacr2 Protein, hydroxycarboxylic acid receptor 3 Protein, HCAR3 Protein, hcar3 Protein
- Background
- Receptor for 3-OH-octanoid acid mediates a negative feedback regulation of adipocyte lipolysis to counteract prolipolytic influences under conditions of physiological or pathological increases in beta-oxidation rates. Acts as a low affinity receptor for nicotinic acid. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet.[UniProtKB/Swiss-Prot Function]
- Molecular Weight
- The human full length HCAR3 protein has a MW of 44.5kDa
- UniProt
- P49019
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