GRM8 Protein

Catalog No. ABIN7538306

Product Details

Target: GRM8 (Glutamate Receptor, Metabotropic 8 (GRM8))

Protein Type: Synthetic Nanodisc

Origin: Human

Source: Mammalian Cells

Purpose: Human GRM8 full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Comment:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: GRM8 (Glutamate Receptor, Metabotropic 8 (GRM8))

Alternative Name: GRM8 (GRM8 Products)

Synonyms: mGluR8 Protein, GRM8 Protein, MGLUR8 Protein, GLUR8 Protein, GPRC1H Protein, mGlu8 Protein, A230002O04 Protein, Gprc1h Protein, Glur8 Protein, Mglur8 Protein, mGluR8b Protein, mGlur Protein, glutamate metabotropic receptor 8 Protein, metabotropic glutamate receptor 8 Protein, glutamate receptor, metabotropic 8 Protein, GRM8 Protein, MGLUR8 Protein, LOAG_14062 Protein, Grm8 Protein

Background: L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Molecular Weight: The human full length GRM8 protein has a MW of 101.7kDa

UniProt: O00222

Pathways: cAMP Metabolic Process, Synaptic Membrane