Metabotropic Glutamate Receptor 6 Protein

Catalog No. ABIN7538305

Product Details

Target: Metabotropic Glutamate Receptor 6 (GRM6) (Glutamate Receptor, Metabotropic 6 (GRM6))

Protein Type: Synthetic Nanodisc

Origin: Human

Source: Mammalian Cells

Purpose: Human GRM6 full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Comment:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Metabotropic Glutamate Receptor 6 (GRM6) (Glutamate Receptor, Metabotropic 6 (GRM6))

Alternative Name: GRM6 (GRM6 Products)

Synonyms: mGluR6 Protein, CSNB1B Protein, GPRC1F Protein, MGLUR6 Protein, mGlu6 Protein, GRM6 Protein, BC021919 Protein, Gm3 Protein, Gprc1f Protein, nerg1 Protein, nob2 Protein, nob3 Protein, nob4 Protein, si:dkey-52h10.3 Protein, cb496 Protein, grma Protein, zK13A21.16 Protein, zgc:158329 Protein, glutamate metabotropic receptor 6 Protein, glutamate receptor, metabotropic 6 Protein, glutamate receptor, metabotropic 6a Protein, glutamate receptor, metabotropic 6b Protein, Grm6 Protein, GRM6 Protein, grm6a Protein, grm6b Protein

Background: L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Mutations in this gene result in congenital stationary night blindness type 1B. [provided by RefSeq, May 2018]

Molecular Weight: The human full length GRM6 protein has a MW of 95.5kDa

UniProt: O15303